Study of structural changes of gluten proteins during bread dough mixing by Raman spectroscopy
نویسندگان
چکیده
The aim of the present study was to evaluate Raman spectroscopy in determining changes that occur structure gluten proteins induced during bread dough mixing. spectra were measured directly within dough. Three particular phases mixing studied: under-mixing, optimum and over-mixing. A thiol blocking reagent, Tris(2-carboxyethyl)phosphine (TCEP) then used reduce disulphide bonds confirm important role bridges network formation. For control dough, most occurred phase when an increase intermolecular bonds, anti-parallel ?-sheet ?-helix structures observed, combined with hydrophobic burial tryptophan tyrosine residues. addition TCEP appeared effectively formation lead a more disordered secondary protein structure.
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2021
ISSN: ['1873-7072', '0308-8146']
DOI: https://doi.org/10.1016/j.foodchem.2021.129916